Cultrex RGF Basement Membrane Extract, Type 2 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex RGF Basement Membrane Extract, Type 2
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3533-005-02
Quantity5 mL
Brand/TrademarkR&D Systems, a Bio-Techne Brand
ApplicationsBioactivity
Format12 - 18 mg/mL
SpeciesMouse
TypeBasement Membrane Extract
StorageProduct is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 µg/ml gentamicin sulfate. Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) BME-free primary patient-specific organoids obtained with a one-day mimicking method to replicate the corresponding tumor for personalized treatment optionsFront OncolDecember 15, 2023Yan Zhu, Zhechun Ding, Yini Wang, Qing Wu, Dongmei Chen, Luanhong Wang, Yuancheng Li, Yao Yao, Jiman Huang, Yun Li, et al.Gibco, USA) for 2 min to single cells, then 3-5 single cells were gathered and embedded in the BME (3533-005-02, R&D Systems, USA) for about 2 weeks. The spheroids with the diameters during 200 μm to 500 μm were

(2) PATJ inhibits histone deacetylase 7 to control tight junction formation and cell polarityCellular and Molecular Life SciencesNovember 1, 2023Julia Fiedler, Thomas Moennig, Johanna H. Hinrichs, Annika Weber, Thomas Wagner, Tim Hemmer, Rita Schröter, Thomas Weide, Daniel Epting, Carsten Bergmann, et al.slides (ibidi, #80826), pre-coated with 8 µl Cultrex Basement Membrane Extract Type 2 (Bio-Techne, #3533-005-02). Cells were trypsinized and a cell suspension at 20,000 cells/ml was prepared in a growth medium containing

(3) Integration of Patient-Derived Organoids and Organ-on-Chip Systems: Investigating Colorectal Cancer Invasion within the Mechanical and GABAergic Tumor MicroenvironmentbioRxiv : the preprint server for biologySeptember 17, 2023Strelez C, Perez R, Chlystek JS, Cherry C, Yoon AY, Haliday B, Shah C, Ghaffarian K, Sun RX, Jiang H, et al.

(4) Advancing Cancer Therapy Predictions with Patient-Derived Organoid Models of Metastatic Breast CancerCancers (Basel)July 13, 2023Cansu E. Önder, Teresa J. Ziegler, Ronja Becker, Sara Y. Brucker, Andreas D. Hartkopf, Tobias Engler, André KochCultrex Reduced Growth Factor Basement Membrane Extract, Type 2 Select, Bio-techne, Minneapolis, MN, USA, 3533-005-02) at a ratio of 30% cell suspension to 70% BME. Twenty µL droplets were plated out on 48-well plates

(5) RAD51 Foci as a Biomarker Predictive of Platinum Chemotherapy Response in Ovarian CancerClinical Cancer ResearchJuly 5, 2023Amanda J. Compadre, Lillian N. van Biljon, Mark C. Valentine, Alba Llop-Guevara, Emily Graham, Bisiayo Fashemi, Andrea Herencia-Ropero, Emilee N. Kotnik, Isaac Cooper, Shariska P. Harrington, et al.420301). Single-cell suspensions of tumor or ascites were resuspended in 75% Cultrex (R&D Systems; No. 353300502) and 25% organoid base media [Advanced DMEM/F12 (Thermo Fisher Scientific; No. 12634028) supplemented

(6) TMEM123 a key player in immune surveillance of colorectal cancerFrontiers in ImmunologyJune 23, 2023Elisa Pesce, Chiara Cordiglieri, Mauro Bombaci, Serenella Eppenberger-Castori, Stefania Oliveto, Cristina Manara, Mariacristina Crosti, Caner Ercan, Mairene Coto, Andrea Gobbini, et al.,GE Healthcare,Cat# RPN2236 Basement membrane extract (BME; Cultrex PC BME RGF type 2),R&D systems (Biotechne),Cat# 3533-005-02 Colon carcinoma tissue array (TMA),Biomax us,Cat# CO1004 PrestoBlue™ Cell Viability Reagent,Invitrogen

(7) Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9STAR ProtocolsMarch 1, 2023Anne Huber, Christine Dijkstra, Matthias Ernst, Moritz F. Eissmann11836170001 "Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear (RGF BME)",R&D Systems,Cat# 3533-005-02 DPBS,Thermo Fisher Scientific,Cat# 14190144 Fetal bovine serum (FBS),Bovogen biologicals,Cat# SFBS-AU Gentle cell dissociation reagent

(8) Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9STAR ProtocolsMarch 1, 2023Dedrick Kok Hong Chan, Scott David Collins, Simon James Alexander Buczacki430167 "Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear",Bio-Techne,Cat# 3533-005-02 Cultrex Organoid Harvesting Solution,Bio-Techne,Cat# 3700-100-01 Transfer pipettes,VWR,Cat# 612-4491 Dragonfly

(9) γδ T cells are effectors of immunotherapy in cancers with HLA class I defectsNatureJanuary 1, 2023de Vries NL, van de Haar J, Veninga V, Chalabi M, Ijsselsteijn ME, van der Ploeg M, van den Bulk J, Ruano D, van den Berg JG, Haanen JB, et al.IV (Sigma-Aldrich) and 10 μM Y-27632 (Sigma-Aldrich). Cells were embedded in Cultrex RGF BME type 2 (3533-005-02, R&D systems) and placed into a 37 °C incubator for 20 min. Human CRC organoid medium is composed of

(10) Identifying drivers of breast cancer metastasis in progressively invasive subpopulations of zebrafish-xenografted MDA-MB-231Molecular BiomedicineDecember 1, 2022Jerry Xiao et al.rat tail Collagen I/BM mix (2.4 mg/mL Collagen I (Corning #354236) and 2 mg/mL Cultrex (BioTechne #3533–005-02), plated on 20 uL of a base layer of Collagen I/Cultrex, overlaied with serum free media, and allowed

(11) Re-purposing the pro-senescence properties of doxorubicin to introduce immunotherapy in breast cancer brain metastasisCell Reports MedicineNovember 15, 2022Rebeca Uceda-Castro, Andreia S. Margarido, Lesley Cornet, Serena Vegna, Kerstin Hahn, Ji-Ying Song, Diana A. Putavet, Mariska van Geldorp, Ceren H. Çitirikkaya, Peter L.J. de Keizer, et al.washed and spun down, it was resuspended in Basal Membrane Extract (BME) type 2 (R&D systems Cat. No. 3533-005-02) diluted at a 2:1 ratio with DMEM/F12 GlutaMAX medium (Thermo Fisher Scientific, Cat. No. 10565018).

(12) Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex VivoJournal of Visualized ExperimentsOctober 31, 2022Amna N. Naser, Qun Lu, Yan-Hua Chen(24x50–1.5),Fisher Scientific,12544E Cryomolds,vwr,25608-916 "Cultrex RCF BME, Type 2",R&D Systems,3533-005-02 Dewar Flask,Thomas Scientific,1173F61 DMEM High Glucose with L-Glutamine,ATCC,30-2002 1X Dulbecco’s

(13) A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editingNature communicationsOctober 28, 2022Vizoso M, E J Pritchard C, Bombardelli L, van den Broek B, Krimpenfort P, Beijersbergen RL, Jalink K, van Rheenen J.

(14) Mesenchymal tumor organoid models recapitulate rhabdomyosarcoma subtypesEMBO molecular medicineOctober 10, 2022Meister MT, Groot Koerkamp MJA, de Souza T, Breunis WB, Frazer-Mendelewska E, Brok M, DeMartino J, Manders F, Calandrini C, Kerstens HHD, et al.0.1–0.5% BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533‐005‐02). We observed batch‐to‐batch variations of this product, resulting occasionally in suboptimal attachment

(15) Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cellsNature communicationsSeptember 19, 2022Adnani L, Kassouf J, Meehan B, Spinelli C, Tawil N, Nakano I, Rak J.C57bl/6 (Charles River Labs) mice were collected and ~1 mm rings were cut and placed in the BME matrix (3533-005-02, Cultrex RGF BME, Type 2, R&D Systems) in a 12-well dish. After the ring solidified in the BME, aortic

(16) WNK1 collaborates with TGF-β in endothelial cell junction turnover and angiogenesisProceedings of the National Academy of Sciences of the United States of AmericaJuly 26, 2022Jaykumar AB, Plumber S, Barry DM, Binns D, Wichaidit C, Grzemska M, Earnest S, Goldsmith EJ, Cleaver O, Cobb MH.pieces. Meanwhile, 48-well plates were coated with 150 µL of Cultrex RGF BME matrix type 2 (R&D Systems, 3533–005-02) or 200 µL of collagen type I (Fisher Scientific, CB354249). The plates were left to solidify the matrix

(17) Epithelial-to-Mesenchymal Transition Drives Invasiveness of Breast Cancer Brain MetastasesCancersJune 25, 2022Margarido AS, Uceda-Castro R, Hahn K, de Bruijn R, Kester L, Hofland I, Lohuis J, Seinstra D, Broeks A, Jonkers J, et al.organoids were cultured in drops of 50 μL of Basal Membrane Extract (BME) type 2 (R&D systems, Cat. No. 3533-005-02) diluted at a 2:1 ratio with DMEM/F12 GlutaMAX medium (Gibco, Cat. No. 10565018) in a pre-warm 24-well

(18) Sorafenib and nitazoxanide disrupt mitochondrial function and inhibit regrowth capacity in three-dimensional models of hepatocellular and colorectal carcinomaScientific reportsMay 27, 2022Ek F, Blom K, Selvin T, Rudfeldt J, Andersson C, Senkowski W, Brechot C, Nygren P, Larsson R, Jarvius M, et al.suspended in ice-cold Cultrex® Basement Membrane Extract (BME) gel (10 mg/ml in PBS (D8537, Sigma-Aldrich) (3533-005-02, Bio-Techne) and seeded in 20 μl droplets in 6-well plates (Nunclon Delta 140685, Thermo Fisher Scientific)

(19) TRAP1 regulates the response of colorectal cancer cells to hypoxia and inhibits ribosome biogenesis under conditions of oxygen deprivationInternational Journal of OncologyMay 6, 2022Giuseppina Bruno et al.Following the removal of the virus-containing medium, organoids were plated in ~50 µl BME (cat. no. 353300502, Bio-Techne Corporation). Transduced organoids were grown in Advance DMEM/12 full of growth factors

(20) FER regulates endosomal recycling and is a predictor for adjuvant taxane benefit in breast cancerCell reportsApril 5, 2022Tavares S, Liv N, Pasolli M, Opdam M, Rätze MAK, Saornil M, Sluimer LM, Hengeveld RCC, van Es R, van Werkhoven E, et al.

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Cultrex RGF Basement Membrane Extract, Type 2 from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Cultrex RGF Basement Membrane Extract, Type 2 from R&D Systems, a Bio-Techne Brand

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